News

  • 1
  • 1

What is the difference between RT-PCR, QPCR, and Real-time PCR?

Jime

What is the difference between RT-PCR, QPCR, and Real-time PCR?

What is RT-PCR?

RT-PCR is reverse transcription PCR (reverse transcription PCR) or reverse transcription-PCR (RT-PCR), which is a widely used variant of polymerase chain reaction (PCR). In RT-PCR, an RNA strand is reverse transcribed into complementary DNA, which is then used as a template for DNA amplification by PCR.

What is qPCR, Real-time-PCR?

In fact, Realtime-PCR and qPCR (Quantitative Rea-ltime-PCR) are the same thing, both are real-time quantitative PCR, which means that each cycle in the PCR process has a real-time record of data, so the number of starting templates can be analyzed. Precise analysis.

Although Real-time PCR (real-time fluorescent quantitative PCR) and Reverse transcription PCR (reverse transcription PCR) seem to be abbreviated as RT-PCR. However, the international convention is: RT-PCR refers specifically to reverse transcription PCR, Real-time PCR is generally abbreviated as qPCR (quantitative real-time PCR).

What is real-time RT-PCR (or RT-qPCR)?

The "RT" in the middle of real-time RT-PCR is reverse transcription (reverse transcription), and the whole meaning is the real-time reverse transcription PCR technology (quantitative Real-time RT-PCR) using mRNA or total RNA as a template. Real time RT-PCR refers to the combination of qPCR + RT-PCR, which means that mRNA is reverse transcribed into cDNA and then used as a template for real-time fluorescent PCR analysis, because RT-PCR can be qualitative, but not quantitative detection. .

Therefore, it is not difficult to understand that real-time RT-PCR (RT-qPCR) is a reverse transcription PCR combined with fluorescence quantitative technology: first, cDNA (RT) is obtained from RNA reverse transcription, and then quantitative analysis is performed by Real-time PCR ( qPCR).

Therefore, real-time fluorescence quantitative PCR, it can not only use cDNA as a template, but also use genomic DNA as a template.

 

Professional manufacturer of Nucleic Acid (DNA & RNA) Extraction and Analysis products supplier

GENETURE is a group company,we own two factories: Ascend and Dianrun,to provide one stop solution of Nucleic Acid Extraction and Analysis,including solution for COVID-19. Main products including: Nucleic acid extraction or purification kit,Automatic nucleic acid extractor, PCR system, PCR kit, Magnetic beads, and lab consumables of 96 well deep plate,Magnetic rod comb,PCR tube,PCR plate,Pipette tips,centrifuge tubes. etc. Geneture proteinase K also meets the needs of PCR technology. Please feel free to contact us.

 

Inquiry us

  • tags

Our Latest News

What is Nano Iron Oxide Powder

During the past few years, nano iron oxide has increased to the forefront of materials research. Its various applications vary from antimicrobial representatives to drivers and regenerative medicine. The residential properties of iron oxide nanoparti…

The Properties of 18Ni300 Alloy

The microstructures of 18Ni300 alloy18Ni300 is a more powerful metal than the various other sorts of alloys. It has the most effective durability and tensile toughness. Its stamina in tensile and outstanding longevity make it a wonderful choice for a…

Relative Atomic Mass of Manganese Dioxide

Determining the family member atomic mass of manganese dioxide is extremely crucial. This is important due to the fact that this material is exceptionally harmful as well as can create a variety of unfavorable health effects. The following is a quick…